Meren reddy biography of michael

Abstract

Cholera toxin (CT) is the prototype for the Vibrio cholerae-Escherichia coli family of heat-labile enterotoxins having an AB5 structure. By substituting amino acids in the enzymatic A subunit that are highly conserved in all members of this family, we constructed 23 variants of CT that exhibited decreased or undetectable toxicity and we characterized their biological and biochemical properties. Many variants exhibited previously undescribed temperature-sensitive assembly of holotoxin and/or increased sensitivity to proteolysis, which in all cases correlated with exposure of epitopes of CT-A that are normally hidden in native CT holotoxin. Substitutions within and deletion of the entire active-site-occluding loop demonstrated a prominent role for His-44 and this loop in the structure and activity of CT. Several novel variants with wild-type assembly and stability showed significantly decreased toxicity and enzymatic activity (e.g., variants at positions R11, I16, R25, E29, and S68+V72). In most variants the reduction in toxicity was proportional to the decrease in enzymatic activity. For substitutions or insertions at E29 and Y30 the decrease in toxicity was 10- and 5-fold more than the reduction in enzymatic activity, but for variants with R25G, E110D, or E112D substitutions the decrease in enzymatic activity was 12- to 50-fold more than the reduction in toxicity. These variants may be useful as tools for additional studies on the cell biology of toxin action and/or as attenuated toxins for adjuvant or vaccine use.

The massive diarrhea characteristic of the disease cholera is in large part due to the action of cholera toxin (CT), produced by Vibrio cholerae of serogroup O1. A better understanding of the structure and function of CT will provide new insights into the pathogenesis of cholera and may aid in the design of safe

Justin Strydom is a South African actor who is well known to South African audiences for his performances in local television series, feature films, and short films. Justin has been in the film and entertainment industry for over 26 years and is probably most recognized as a SAFTA award-winning actor in South African daily drama’s like Isidingo, Generations, 7de Laan and High Rollers; During more than 26 years he has also built up a large body of work both as a writer, director, and producer has contributed to the development and production of almost 30 projects.

Justin has also been involved in training and development of some of South Africa’s new and emerging performance talents like Thabo Malema, Thomas Gumede, Litha Booi, Meren Reddy, Thapelo Mokoena, Mapula Mafole, Oros Mampofu, and many others during his tenure as head of Live Performance at AFDA in Johannesburg (2002 – 2004) and (2012 -2014).

Similarly, he has represented AFDA at numerous South African arts festival workshops. Whilst at AFDA, he attained his honours degree at AFDA Johannesburg (cum laude).

Justin is a passionate and committed performance lecturer and coach, who has inspired many young actors with his energy, honesty, and enthusiasm for the performing arts and his students. He is a very likable character, an excellent collaborator, and is well respected by both his colleagues, peers, and students.

During more than 26 years he has also built up a large body of work both as a writer, director, and producer has contributed to the development and production of almost 30 projects.

2017 American biographical drama television miniseries

Madiba is a three-part American biographicaldrama television miniseries documenting the true lifelong struggle of Xhosa human rights activist, lawyer, political prisoner, and eventual president of South AfricaNelson Mandela to overthrow the oppressive regime of institutionalized racism and segregation known as apartheid. The series stars Laurence Fishburne, Orlando Jones, David Harewood, Michael Nyqvist, Terry Pheto, Jason Kennett and Kate Liquorish. The three-part miniseries made its debut on BET on February 1, 2017, concluding on February 15, 2017.

While the miniseries is named for and largely follows the story of Madiba (Nelson Mandela), Laurence Fishburne has stated that one of the primary intentions of the miniseries was to highlight the important role played by Mandela's many family members, friends, and colleagues at the ANC who worked tirelessly alongside him to successfully overthrow the regime of apartheid.

Cast

South African supporting actors:

• Paul Davies as a police officer
• Nick Els as a police officer
• Frans Linde as a police officer
• Albert van Vuuren as a police officer
• Albert van Vuuren as a SABC cameraman

Episodes

Production

Principal photography began on April 4, 2016, and took place in Cape Town, Johannesburg, and Robben Island, South Africa.

References

External links