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Computational design and investigation of the monomeric spike SARS-CoVferritin nanocage vaccine stability and interactions
Introduction
More than million people have been affected, and over six million have died due to the global coronavirus disease (COVID) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (Acuti Martellucci et al., ; World Health Organization, ; Wu et al., ). SARS-CoV-2 is a single-stranded RNA virus with a positive sense and large RNA genome, four structural proteins, 16 nonstructural proteins, and 911 accessory proteins (Alexandersen et al., ; Malik, ). The four structural proteins are spike, envelope, membrane, and nucleocapsid proteins, with the spike glycoprotein (S protein) being particularly important as it is a prominent coronavirus vaccine target (Andersen et al., ; Choi et al., ; Du et al., ; Hu et al., ; Walls et al., ; Wrapp et al., ). SARS-CoV-2 enters cells by binding to the host cellular receptor Angiotensin-Converting Enzyme 2 (ACE2) via its spike protein (Song et al., ; Zhao et al., ; Piplani et al., ; Jackson et al., ), and typically causes a lower respiratory tract infection, which can progress to severe acute respiratory syndrome and potentially multiple organ failure (Ganji et al., ; Tu et al., ; Tao et al., ; Yang et al., ). The S protein monomer, initially produced as a single polypeptide chain, is made up of a fusion peptide, two heptad repeats, an intracellular domain, an N-terminal domain, two subdomains, and a transmembrane region (Figure 1). After translation it is cleaved into S1 and S2 subunits (Li et al., ; Gui et al., ). A 25 KDa Receptor Binding Domain (RBD; residue range ) within S1 interacts with the ACE2 receptor (Hoffmann et al., ; Walls et al., ). As a result, the S protein dictates SARS-COV-2s infectivity and transmissibility (Du et al., ; Yang et al., ).
Figure 1. Structural representation of the monomeric spike SARS-CoV-2 ectodomain. (A) monomeric spi
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name image description occupation date of birth date of death place of birth place of death wikidata item site links Ayesha Kamasani Iranian muhaddith and jurist Merv Q 1 Razieh Mihaniyeh Iranian Khorasani muhaddith and Sufi ولایت خبوشان Q 2 Afifa Farfani Muslim scholar, narrator, jurist and modernist 'ālim Q 5 Esmat Bukhari Iranian poet poet
writer Q 4 Amina Qazvini Iranian poet, religious scholar poet
writer Qazvin Q 2 Jahan Ara Kurdish poet poet
writer Paveh Javanrud Q 0 Touran Agha Khanoum Qajar Iranian poet; daughter of Naser al-Din Shah Qajar and Khazen al-Douleh (–) poet
writer
calligrapher Tabriz Tehran Q 4 Marzieh Zarabi Iranian journalist journalist
author Bandar-e Anzali Q 1 Nimtaj Salmasi Iranian Azeri poet (–) poet
writer
Salmas Tehran Q 4 Mari-Hranuysh Felekyan actress actor Tehran Q 1 Mariam Savadkukhi Reza Shah Pahlavi's wife consort Q 2 Badr al-Maluk Bammad Iranian journalist, teacher, social activist (–) teacher
journalist
writer
social activist
pedagogue Q 1 Satenik Aghababian Iranian actress, opera singer (–) actor
opera singer Tehran Q 3 Justina Iranian hiphop artist rapper 20th century Tehran Q 3 Fatemeh Sayyah Iranian essayist (–) essayist
Moscow Q 2 Esmat Safavi Iranian actress (–) actor Tehran Tehran Q 1 Haykush Ter-Martirosyan Iranian actress of Armenian descent actor
teacher Tabriz Los Angeles Q 1 Arshi Iranian-Armenian poet poet
writer Tabriz Tehran Q 2 Elbis Farahian Iranian educator, of Armenian descent; known in the field of pre-primary education in Iran teacher
pedagogue Tehran Tehran Q 1 Iran Daftari Iranian actress (–)